American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Sample Questions:

1. A clinical isolate of Klebsiella pneumoniae from a patient with a refractory urinary tract infection exhibits resistance to carbapenems (imipenem, meropenem, ertapenem). PCR analysis reveals the presence of the blaKPC gene. Which of the following enzymatic mechanisms is responsible for this resistance?

A) Modification of the ribosomal target site, preventing antibiotic binding.
B) Hydrolysis of the beta-lactam ring, leading to an inactive antibiotic derivative.
C) Trapping of the carbapenem molecule by a decoy protein.
D) Efflux of the carbapenem antibiotic out of the bacterial cell.

2. A patient presents with a chronic cough, weight loss, and night sweats. Chest X-ray shows cavitarylesions in the upper lobes. Sputum is acid-fast positive. Drug susceptibility testing reveals resistance to isoniazid but susceptibility to rifampin, ethambutol, and pyrazinamide. The MOST appropriate treatment regimen should include:

A) Isoniazid, rifampin, pyrazinamide, and ethambutol
B) Isoniazid and rifampin
C) Isoniazid, ethambutol, and pyrazinamide
D) Rifampin, ethambutol, and pyrazinamide

3. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?

A) Cysteine and pyridoxal
B) Factor V (NAD) only
C) Factor X (hemin) only
D) Both Factor X and Factor V

4. The erm gene in Staphylococcus aureus confers resistance to which class(es) of antibiotics via modification of the ribosomal target site?

A) Macrolides, Lincosamides, and Streptogramin B (MLS<sub>B</sub>)
B) Fluoroquinolones
C) Aminoglycosides
D) Beta-lactams only

5. A patient with a history of recurrent sinusitis and bronchitis is diagnosed with cystic fibrosis. Sputum cultures repeatedly grow a Gram-negative rod that is oxidase-positive and produces a blue-green pigment. This organism's chronic colonization of the respiratory tract in cystic fibrosis patients is primarily due to its ability to:

A) Evade phagocytosis by alveolar macrophages through capsule production.
B) Produce potent exotoxins that damage the ciliated epithelium.
C) Exhibit rapid intracellular replication within respiratory epithelial cells.
D) Form a biofilm that protects it from host defenses and antibiotics.

Solutions: